Mukherjee, D. and Selvi, V.A. and Masto, R.E. (2018) INVESTIGATION OF COAL-METHANOARCHAEAL ASSEMBLAGE IN DEEP SUBSURFACEUNDERGROUND USING ENRICHMENT CULTURE TECHNIQUE. Petroleum and Coal Journal , 60 (5). pp. 951-960. ISSN 1337-7027

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Abstract

Enrichment culture of coal samples from underground mines (Jitpur and Moonidih underground mines) located in Dhanbad region, the Indian state of Jharkhand was tested positive for the presence of living microbial lineage capable of producing methane from coal during energy metabolism under laboratory condition. Accordingly, two gram negative, mesophilic, penicillin G and streptomycin resistant strain (BC /C IMFR-ana-CH4-12 and 14) derived from preferred sites were produce methane from acetate. They assimilated complex proteinaceous substances (yeast extract, beef extract, tryptone, and peptone) and amino acids (alanine, cysteine, and methionine) for energy metabolism. The pH ranges for growth were 6.0-7.5 for strain BC /C IMFR-ana-CH4-12 and 6.5-8.5 for strain BC /C IMFR-ana-CH4-14, with the fastest growth at pH 7.5 for BC /C IMFR-ana-CH4-12 and pH 7.0 for strain BC /C IMFR-ana-CH4-14. During growth, doubling time was observed 13.702- 14.005 hours under the optimal condition, and specific growth rate was recorded 0.021/hour for both strains. Based on the study strains were belongs to Methanococcus spp. and Methanobacterium spp., associated with numerous aerobic and anaerobic microbial community in deep subsurface coal deposits as suitable habitat. The ecological implication of their habitat also discussed. The study suggests that there is intense biogenic methane generation activity in a selected environment which may me enhance by stimulating the activity of existing methanoarchaeal consortia.

Item Type:	Article
Uncontrolled Keywords:	Coal bed; Archaea; Methane; Habitat; Substrate; Growth.
Subjects:	Enviornmental Management
Divisions:	UNSPECIFIED
Depositing User:	Mr. B. R. Panduranga
Date Deposited:	08 Nov 2018 04:20
Last Modified:	08 Nov 2018 04:20
URI:	http://cimfr.csircentral.net/id/eprint/1922

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