Kumar, Birendra (2012) Study of the Rate of Removal of Eutrophic Nutrient (Phosphate) by Free and Immobilized Cells of Bacillus Subtilis 2620. Indian Journal of Environmental Protection, 32 (5). pp. 353-360. ISSN 0253-7141

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Abstract

Eutrophication of natural waters is one of the most significant causes of a decline in water quality. Phosphate and nitrate are the 2 major agents of eutrophication. Phosphate when present in appropriate amount plays an important role in maintaining the ecology of the water body but it is hazardous to human health when present in excess. In the present investigation, the phosphate uptake potential by Bacillus subtillis 2620 has been studied. In the experiments, the cells were exposed to various graded concentration of phosphate in the range of 10 to 100 ppm, the uptake potential remained in the range of 86% at 10 ppm to 79% at 100 ppm with a maximum phosphate uptake observed at 50 ppm (91%) and 60 ppm (92.4%) after a week of incubation at 37°C at 150 rpm. However, plating results showed that the cells can tolerate upto 1000 ppm phosphate concentration but uptake at such high concentration were insignificant. The effect of pH on uptake potential showed maximum uptake at pH 5 and 7. However, immobilized cells of Bacillus subtilis 2620 showed effective uptake of about 63% even at 150 ppm after 4 day of incubation under similar conditions as for free cells.

Item Type:	Article
Uncontrolled Keywords:	Eutrophication.
Subjects:	Envieronmental Management Group
Divisions:	UNSPECIFIED
Depositing User:	Dr. Satyendra Kumar Singh
Date Deposited:	14 Jul 2012 05:24
Last Modified:	14 Jul 2012 05:24
URI:	http://cimfr.csircentral.net/id/eprint/1232

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